Synaptophysin can be used to label normal neuroendocrine cells of the human adrenal medulla, skin, carotid body, thyroid, pituitary gland, pancreas, lung, and gastrointestinal mucosa, as well as Paneth’s cells of the gastrointestinal tract and the gastric parietal cells. It may also be used to label neurons in the retina, brain, and spinal cord. It can react with neuroendocrine neoplasms of the neural and epithelial types, such as ganglioneuromas, neuroblastomas, pheochromocytomas, ganglioneuroblastomas, non-chromaffin paragangliomas, and chromaffin.
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Synaptophysin does not have a clone, and the immunogen is the synthetic peptide of the human synaptophysin. The isotype is the Rabbit IgG, and it has an undetermined epitope with a molecular weight of 38kDa.
Applications
Synaptophysin can be used with Immunohistochemistry applications. To prepare your specimen, use Formalin-fixed or paraffin-embedded tissues. Deparaffinized slides are necessary and can be achieved with an alternative to xylene or graded alcohols.
When choosing the concentrated version of the antibody, you should dilute it using a ratio of 1:300. You can also find pre-diluted options available. If your protocol requires a different dilution ratio, you will need to use the concentrated format.
To retrieve the antigen, you can boil tissue sections in a 10mM Citrate buffer with a pH of 6.0. Let it boil for 10 minutes and allow 20 minutes for it to cool to room temperature. Likewise, an incubation period of 10 minutes is required while at room temperature.
You should wash your slides between steps using a PBS/0.05% Tween solution.
The positive control is the pheochromocytoma or pancreas with cellular localization occurring in the Cytoplasm.
Synaptophysin can be used to test for a variety of problems, which can move forward your research and help learn more about the human body and problems. Visit Spring Bioscience now for more information.
