The FOXP3 antibody is part of the winged-helix/forkhead family of transcriptional regulators. It is required for normal immune homeostasis and is expressed at high levels for positive regulatory T-cells in CD25+CD4, as well as at low levels in CD25 negative and CD4 positive cells. It is entirely absent in CD8 positive and CD4 negative T-cells. It is considered to be a master regulatory gene and a better marker for regulatory T-cells.
The FOXP3 is a polyclonal antibody, which means it has no known clone. It is intended to be used for research, and its immunogen is the synthetic peptide that’s derived from the c-terminus of the human protein by the same name. The isotype is the Rabbit IgG with an undetermined epitope. Likewise, it has a molecular weight of 50kDa.
The FOXP3 antibody can be used for Immunohistochemistry applications. To prepare the specimen, you must use a formalin-fixed or paraffin-embedded tissue. Deparaffinized slides are required, which can be cleaned with xylene or its alternative, as well as graded alcohols.
You’ll find a concentrated version and pre-diluted version. If you choose to purchase the concentrated antibody, you will need to dilute it using a ratio of 1:200, though this is an estimate.
To retrieve the antigen, you will need to boil the tissue section in 1mM EDTA with a pH of 8.0 for at least 10 minutes. Let it cool for 20 minutes to get to room temperature. Then, you should incubate it for 30 minutes, while remaining at room temperature.
You should rinse the slides between steps using PBS-Tween.
The positive control is the tonsil, and the cellular localization will happen in the nucleus.
The FOXP3 antibody can help you with your research. Visit Spring Bioscience to learn more about it or to find out how to purchase it.