What Synaptophysin Does And How To Use It

by | Jan 20, 2017 | Science and Technology

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The Synaptophysin antibody can be used to label normal neuroendocrine cells from the human adrenal medulla, skin, pituitary gland, carotid body, thyroid, pancreas, lung, gastrointestinal mucosa and more. It can also be used to label neurons in the spinal cord, brain, and retina. The anti-synaptophysin antibody will react with the neuroendocrine neoplasms of epithelial and neural types, including the chromaffin, neuroblastomas, ganglioneuromas, ganglioneuroblastomas, and more.

More Information

It is designed to be used for research and does not have a clone at this present time. It is considered a synthetic peptide for the human synaptophysin and its IG isotype is the Rabbit IgG. It has an undetermined epitope with a molecular weight of 38kDa.

It has also been tested on humans and recognizes all proteins with a weight of 38kDa. It is designed for use with Immunohistochemistry applications with a particular procedure process. You should only use paraffin-embedded or Formalin-fixed tissues and deparaffinize your slides using xylene or its alternative.

This product comes in a concentrated formula as well as others. If using the concentrated version, you should dilute it with a ratio of 1:300, which is an estimate.

You can retrieve your antigen by boiling your tissue sections with a 10mM citrate buffer with a pH of 6.0 for approximately 10 minutes, allowing to cool to room temperature for at least 20 minutes, with an incubation period of 10 minutes more at room temperature.

How It Can Be Purchased

You can find the Synaptophysin antibody in a 1.0ml, 0.5 ml, and 0.1 ml option in a PBS/1 percent BSA buffer with a pH of 7.6. You can also find it in a pre-diluted form in 7.0 ml.

Synaptophysin is an excellent antibody to use for labeling many neuroendocrine cells from many areas of the body. Visit Spring Bioscience to find out more.